Year
|
Phase
|
Objective
|
Activities
|
Results |
2013
|
Unique
|
1. The study of chemical
systems with controlled functionality
|
1.1. Design, synthesis and testing of the nanoscale systems for
controlled drug releas.
|
1 ISI
paper
|
1.2. Design, synthesis and testing of nanocarrier systems and
nanoscavanger type.
|
3 ISI
papers
|
1.3. Design, synthesis and testing of carriers with
stimuli-sensitive polymers.
|
1 ISI
paper
1 participation
in scientific meeting
|
1.4. Design, synthesis and testing magnetic nanocarrieri with
medical application.
|
2 ISI
papers
|
2. The study of
self-assembly systems for bio macromolecular recognition
|
2.1. Design and synthesis of self-assembling systems able to
detect proteins.
|
1 ISI
paper
1 research
stage
|
2.2. Design and synthesis of self-assembling systems able to
associate with nucleic acids.
|
1 ISI
paper
3 research
stages
|
3. Study of
nano- and micro-chemical architecture for interaction with nucleic acids in
cell cultures
|
3.1. Synthesis and preliminary testing of nanoentities with
fullerene core.
|
1 ISI
paper
|
3.2. Synthesis and preliminary testing of multifunctional micro /
nano-particles based on biopolymers.
|
1 ISI
paper
|
3.3. Study and design of a system with controlling of the
dimensions of the aggregates for transfection applications.
|
1 patent
application OSIM Bucharest
1 ISI
paper
|
4. Study
and preliminary testing of a system class biomimetic tissue substitutes
|
4.1. Synthesis and characterization of a inorganic matrix.
|
1 ISI
paper
1 participation
in scientific meeting
|
2014
|
Unique
|
1. The
study of chemical systems with controlled functionality
|
1.1. Proiectarea, realizarea si testarea de sisteme la nanoscara,
purtatoare de acizi nucleici si active in eliberarea controlata.
|
9 ISI
papers
3 participations
in scientific meetings
4 research
stages
|
1.2 Sinteza unor
sisteme de tip hidrogel cu feed-back prin pH si temperatura.
|
2. Design
and synthesis of biomimetic systems for transfection
|
2.1. Synthesis and testing of squalene derivatives active as
non-viral vectors in circulating nucleic acids.
|
2.2. Obtaining and testing nanoconjugate with magnetizable core.
|
2.3. Synthesis and testing of systems with fullerene core capable
for transfection.
|
2.4. Synthesis of systems capable of self-assembly in the presence
of nucleic acids.
|
2015
|
Unique
|
1. The design and
development of biomimetic systems for transfection
|
1.1. Development and testing of
structured hydrogels, able to load and transport nucleic acids
|
10 ISI
papers
10 participations
in scientific meetings
|
2. Assessment of
transfection ability of the systems developed by the project
|
2.1.
Evaluation of complexing capacity of the non-viral vectors |
2.2. Testing
of the transfection systems in cell culture |
3. Development of
electrochemical testing protocols for the nanoscale systems having
transfection ability
|
3.1.
Electrochemical quantitation of the nucleic acids loaded by the transfection
systems |
4. The design and development of biomimetic systems for
transfection
(preliminaries related to the
2016 stage)
|
4.1. Synthesis
and characterization of biomimetic injectable composites |
4.1.1. Synthesis and characterization of
pH and temperature responsive nanoparticles based on cathionic pullulan
derivatives
|
4.1.2. Synthesis and characterization of
pH and temperature responsive
NIPAM-AM copolymers able to act as nano-containers
in biomedical applications
|
2016
|
Unique
|
1. The design and development of
injectable macromolecular biomimetic matrices for transfection
|
1.1. Development and characterization
of injectable structured composites, able to load and transport nucleic acids
|
3 ISI
papers
2 participations
in scientific meetings
1 patent
application OSIM Bucharest
|
2. Assessment of the transfection ability
of the systems developed during the project
|
2.1.
Evaluation of complexing capacity of the non-viral vectors versus DNA |
2.2. Testing
of the transfection systems for cell culturing |
3. The evaluation of transfection ability
of the structured composites loaded with polyplexes
|
3.1. The
evaluation of transfection ability of the structured composites loaded with
polyplexes |
4. Development of electrochemical
protocols for testing the transfecting systems produced during the project
|
4.1. The
electrochemical evaluation of entities able to sustain transfection |
